Sterylglycosides (SGs) are natural derivates of sterols that occur in various plants either as free SGs or as acylated compounds (ASGs). The acylated form is, due to the non-polar fatty acid component, soluble in edible oils like soybean oil, palm oil or rapeseed oil, which are inter alia raw materials for biodiesel production. In biodiesel production, plant oils, or to be more precise, triacylglycerols are in a transesterification reaction with methanol transformed into fatty acid methyl esters (FAME). In this reaction, besides the favored product, various by products occur. One of these by-products are SGs, that are made of acylated SGs, which precipitate during storage of biodiesel. These precipitations can lead to problems like clogging of filters. Due to the high melting point of these compounds, they also have a negative influence on the fluidity of biodiesel, especially at low temperatures, which can lead to engine failures. In this paper, SGs were synthesized in three different ways. Already known methods were modified respectively in literature not yet described methods were used. Next to the description of synthesis, also the advantages and disadvantages of the different methods, concerning the yield, the purity of the products as well as the working hours investigated are discussed. Moreover, the methods are compared regarding the costs of the raw materials which were used. The synthesized SG should be used as model compounds for new methods of analysis. Furthermore, different methods of analysis, like NMR-spectroscopy, MS-TOF as well as gas chromatography are described and utilized. Also the pros and cons of the analysis-methods are pointed out. Moreover, different experiments for enzymatic production of ASGs from SGs were carried out. Besides, rapeseed-fatty acid methyl ester was analyzed by gas chromatography and the content of SGs was determined.