At the present work, triathletes (3 women , 7 men) who are preparing for a long distance triathlon ( swim 3.8 km / 180 km cycling / 42 km run ) were examined. They wanted the basis of endocrine and biochemical parameters such as leptin, ghrelin , insulin, IGFB -3 , Total Anitioxidant status (TAS) and urea explore the effects of a comprehensive training period emphasized on the hormonal status of triathletes . In addition, biometric indicators such as body composition and BMI were collected the athletes to document a possible substance loss of muscle mass and the associated potential overload conditions.Methods: The athletes were tested twice at an interval of eleven weeks. Through the measurements of the ?Lipometer? , the parts were raised from FM to LBM. Furthermore, the chemical parameters are measured in the blood plasma of the athletes. The training documentation (training hours / week ) of the athletes was eleven weeks before the fist measurement and also evaluated eleven weeks after the first examination .Results: The average horrmone concentration in the blood of the examined athletes in leptin decreased significantly ( t = 2.341 * , p < .05 ) and ghrelin highly significant ( t = 13,353 ** , p < .00) . The other recorded values of insulin, IGFB -3 , TAS and urea would be hardly altered . Similarly, FM remained to LBM the constant group.Conclusion: The measured values of leptin and ghrelin suggest an increased quality of the endurance levels of athletes and a good energy balance. The measured results of the biometric and other biochemical parameters indicate that there were no overload conditions of the investigated athletes through the long distance triathlon training.