This thesis investigates the influence of flight simulation experience and quality of mental models on the electrodermal activity (EDA) while flying in a flight simulator. The skin conductance level (SCL) and the number of non-specific skin conductance reactions (NS.SCR) were used as physiological parameters. Testing was conducted with 36 students of the Technical University of Graz (TUG), who studied telematics, computer science and software development. The participants were splitted into groups according to level of experience (a little/a lot) and quality of mental models for each maneuver (bad/good) by the median. The literature shows that both, the experience and the quality of mental models, have an impact on the mental workload which can be measured using the EDA. The evaluation of the hypotheses was performed by using three two-factor multivariate analysis of variance. No significant differences in the NS.SCR and SCL depending on the experience or the quality of the mental models for the different maneuvers were found. However, a significant influence of the quality of mental models for each maneuver on the performance in the maneuver was shown. In addition, a significant difference in the performance-based activation (BSKE) and the general deactivation (BSKE) could be found depending on the time of measurement (before flying/after flying).