"New types of designer drugs" are synthetic substances that are often synthesized in clandestine laboratories and then sold on the internet or in headshops. Through chemical modifications, mostly from illegal substances such as amphetamines or cathinone, they are not affected by law and therefore they can be consumed legally. They represent a major challenge for the monitoring of psychoactive substances. These derivatives possess a chiral centre and occur as two mirror-image forms of the same structure. Usually they exist as a mixture of the two enantiomers (racemate).These substances are often administered by self-medication. There are no strip tests for these substances available. However, the biggest problem is the fact that effects, pharmacology, toxicology, and metabolism of such "new designer drugs" are not or only partly known. In most cases, the information about the profile of action is based on advice.Consequently, it is important to develop methods that allow separation of chiral compounds. Through this separation, it is possible to make an assessment of whether these substances are sold over the internet as a racemate or offered as enantiopure compounds. In this thesis an HPLC method was developed to separate derivatives of amphetamines and cathinone into their enantiomers. A conventional reverse-phase column and a water/methanol mixture were used. As chiral selector, sulfated beta-cyclodextrin dissolved in the mobile phase was used. To optimize the composition of the mobile phase was varied. Under optimal conditions, 11 cathinone derivatives were partially separated, additionally, 6 cathinone derivatives and 7 amphetamine derivatives were baseline separated.