The focus of this Master thesis lies on the transfer of knowledge from research institutions to enterprises, and in particular to small and medium enterprises (SMEs). It is the primarily purpose of such a knowledge transfer to enlarge an enterprise's knowledge base through scientific findings of the research institution in order to strengthen the enterprise's competence to create innovations and to enhance or assure its competitive position. However, it needs some preconditions and incentives on the part of both transfer partners to enable a transfer to take place and to proceed efficiently. Similarly, there exist also barriers, resistances as well as supporting factors within a knowledge transfer which possibly impede or facilitate the diffusion of knowledge, the transfer itself or the adoption of knowledge on the part of the enterprise. Thus, the purpose of this master thesis is the identification and analysis of such barriers, obstacles, resistances and supporting factors in knowledge transfers from research institutions to enterprises, the formulation of applicable, practical implications for action as well as the development of a guideline for practitioners and transfer managers. On the basis of results of previous (empirical) studies 33 impeding and 18 facilitating factors in knowledge transfers were identified and analyzed, whereat 10 influencing factors were to be considered as SME-specific. In addition, factors of particular impact were derived and taken into consideration within the formulation of practical implications and development of the guideline. For the influencing factor's identification and analysis it was reverted to a 3-phase-model of knowledge transfer and, thereby, attempted to assign the determined factors to the three phases of transfer.