Vibrio cholerae is a gram negative, human pathogen and the causative agent of cholera. The natural habitat of V. cholerae are aquatic systems. Infection of the human host occurs via ingestion of contaminated food and water. V. cholerae is motile due to a single polar flagellum and has a chemotaxis system that allows the bacterium to sense environmental signals and respond to them with a change in movement direction. Motility as well as chemotaxis are key factors during colonization of the small intestine, as well as in the process of leaving the host and subsequent spread in the environment. The transcriptional hierarchy of flagellar biosynthesis is composed of four classes based on their temporal expression patterns. The chemotaxissystem consists of transmembrane receptors, a two-component system and a methylation/demethylation system. An important part of the chemotaxis system are the phosphatases of the CheC-superfamily. In V. cholerae no active homologue of the CheC-superfamilie was identified so far. During this work, the transcpritional regulation of flrD was elucidated. Promoter mapping showed one putative s70 promoter and three putative s28 promoters for flrD and the s28 dependency was subsequently confirmed via phosphatase assays. In addition, the open reading frame VC0377 was investigated during this work. Deletion of VC0377 resulted in a hyperswarming phenotype. It was shown that VC0377 has no impact on cell growth. Furthermore, an involvement in flagellar biosynthesis could be ruled out. Accordingly, the observed hyperswarming phenotype is most likely to be attributed to an involvement in chemotaxis, a hypothesis which is supported by bioinformatical predictions, showing VC0377 to be a CheX homologue.