In the present study with six subjects a step test was performed on the bicycle ergometer. Due to the evaluation of the level test subjects completed the endurance tests of 30 minutes at each stage of the testing stage. From these results of the endurance tests lactate-curves have been redesigned and completed with the aid of extrapolation up to maximum values. Lactate-curves are obtained with a virtual stage length from 1 minute to 31 minutes. With the results of recently conducted individual determination of turn-points, analysis of variance was performed, which revealed that there is in relation to the stage length, no significant differences for the evaluation. It was also checked the correlation between Lactate Turn Point 2 and the Maximal Lactate Steady State, and here is found a very strong correlation. In determining the turn-points can be said, based on the results of this study, that an individual determination is preferable to the use of fixed threshold concepts. The three-phase energy supply can be seen in the testing stage, as in the endurance tests.