Abstract In this study, pitch perception was tested in one hundred professional musicians with regard to their played main instruments. The instrumentalists were either pianists, violinists, guitarists, drummers, or vocalists. Two hearing tests, the Auditory Ambiguity Test (AAT, Seither- Preisler et al. 2007) and the ?Schneider- Bleeck- Test? (Schneider et al. 2005), were used to classify subjects as fundamental pitch - and as spectral pitch - listeners. No difference was found amongst the different groups of instrumentalists with regard to their hearing mode. Furthermore, the influence of the musicians? musical audiation (measured by the AMMA-Test) on their pitch perception mode was tested. The instrumentalists? musical activities, as assessed from questionnaire, were used as predictors for the prevalent pitch perception mode. However, no significant influential factors could be identified.