Liquid chromatography is one of the most popular and versatile separation techniques. In the charactzerization of polymers, different approaches like size exclusion chromatography (SEC), liquid adsorption chromatography (LAC) and liquid chromatography under critical conditions (LCCC) can be applied, which separate according to different criteria. The mode of chromatography is determined by the so called interaction parameter, c, which describes the interaction of a polymer with the stationary phase. It is positive in LAC, negative in SEC and equals zero in LCCC. In LAC, the interaction parameter of a given polymer depends on the nature of the stationary phase, the composition of the mobile phase and on temperature. If these dependences are known, chromatographic separations can be simulated and the experimental conditions optimized. Therefore, a data base containing this information can be extremely helpful for chromatographers, as it can save time and solvents in method development. For functional polymers, an additional parameter (which describes the influence of the end groups) must be taken into account. In this thesis, the dependence of the interaction parameter of various polymers on mobile phase composition and temperature was studied on different columns. A method to determine the endgroup parameter has also been developed. From the temperature dependence of retention, the thermodynamics parameters were determined, which are responsible for the chromatographic separation. The results thus obtained were described in several publications in international journals.