To free old species of beans (Phaseolus vulgaris) from virus infection regeneration via organogenesis using tissues from hypocotyl or epicotyl without callus phase was applied. The plant explants were transferred to a medium which consisted of Murashige and Skoog 4.3 g L-1 basal medium and 103 g L-1 vitamins, Microagar and was supplemented with 30 g L-1 sucrose. Different concentrations of benzylaminopurine (BAP) and naphthaleneaceticacid (NAA) were assessed for callus induction and proliferation. Using negative staining method together with electron microscopy, seedling material, calli and plantlets regenerated via organogenesis were investigated and virus infection was proved. Therefore there was no report of a successful producing of virus-free cultures. Because of missing evidence of regeneration of callus cultures of Phaseolus, focus was on determining the environmental conditions like the optimum of concentrations of BAP, NAA and carbon source, light duration, sterilization, and on exploring the general procedures.